The single channel system, for liver, kidney and mesenteric beds, includes the following:
The moist chamber: Including base and cover, it is equipped with stainless steel positioning systems to adjust perfusion cannula or veina positions, without creating constraint on the vessel.
Lateral accesses allow catheters introduction without having to remove cover (Useful to collect discharge of bile in liver perfusion or discharge of urine in kidney perfusion).
The bubble trap located just before the perfusion cannula, has a upper access used for direct bolus injection or to adjust the liquid level, which determines the compliance during the experiment.
Perfusion system: To provide oxygen and nutrients to the isolated organ, it includes a fritted glass, a perfusate reservoir, a peristaltic pump, a pressure/flow regulator, holders and cannulas for veina and artery perfusion.
Heating system: Made of a heat exchanger and a heater/pump, it maintains the organ at the right temperature (usually 37°C). A cooler can be used as well when low temperature are required.
Data acquisition system: transducers, usbACQ interface box and IOX2 software allow to acquire perfusion flow and pressure.
The 4-channel system for mesenteric beds includes the following:
4 organ baths: Equipped with a stainless-steel grid, organ bed height and bath volume are adjustable
Perfusion system: To provide oxygen and nutrients to the isolated organ, it includes 3 peristaltic pumps, and several small cannulas available to suit different artery sizes.
A heating system: To keep the temperature of the perfusate constant.
Data acquisition system: blood pressure transducer, usbAMP amplifier and IOX2 software for pressure acquisition.
A stimulator: if electrical stimulation is required.
An asynchronous stimulator can be optionally delivered with the 4-channel mesenteric beds system. With advanced electrical stimulation features, it generates a constant current or a constant voltage on each of up to 4 channels independently.
Several stimulation parameters can be adjusted, making it possible to create a large variety of stimulation protocols:
The stimulator can be operated manually or remotely from IOX2 acquisition software.
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