The single channel system, for liver, kidney and mesenteric beds, includes the following:
The moist chamber: Including base and cover, it is equipped with stainless steel positioning systems to adjust perfusion cannula or veina positions, without creating constraint on the vessel.
Lateral accesses allow catheters introduction without having to remove cover (Useful to collect discharge of bile in liver perfusion or discharge of urine in kidney perfusion).
The bubble trap located just before the perfusion cannula, has a upper access used for direct bolus injection or to adjust the liquid level, which determines the compliance during the experiment.
Perfusion system: To provide oxygen and nutrients to the isolated organ, it includes a fritted glass, a perfusate reservoir, a peristaltic pump, a pressure/flow regulator, holders and cannulas for veina and artery perfusion.
Heating system: Made of a heat exchanger and a heater/pump, it maintains the organ at the right temperature (usually 37°C). A cooler can be used as well when low temperature are required.
Data acquisition system: transducers, usbACQ interface box and IOX2 software allow to acquire perfusion flow and pressure.