Technique & Measurements

Perfusion

The perfusion system provides oxygen and nutrients to the organ while delivering drugs diluted in the perfusion reservoir to the organ.

Heated water circulates continually through the space behind the bath walls, bathing the heat exchanger through which the perfusate flows. In this way, heat is transferred from the heated water to the perfusate, which is maintained at the right temperature.

Organs can be simply perfused and installed in the chamber without being immersed into the liquid. For applications requiring the organ to be immersed, one channel of the peristaltic pump is used to fill the bath while the second channel is used for overflow.

Carbogen is released directly into the buffer solution.

In the single channel system, the organ can be perfused at a constant flow or a constant pressure rate through the artery. A peristaltic pump is directly controlled by a pressure/flow regulator. Pressure transducer and pump are directly connected to the pressure regulator (No need of extra amplifier) that provide flow and pressure signals. It is also possible to use the pressure regulator connected to veina to adjust the post-charge pressure.

In 4-channel system, mesenteric beds are perfused at constant flow. Pressure is measured just upstream of the cannula, reflecting changes in vascular tone.

Flow rate is directly adjusted by adjusting the speed of the peristaltic pump. This flow is constant and not monitored. The peristaltic pumps provide mesenteric perfusion, bath perfusion and baths overflow (to aspirate excess of liquid going out of the bath due to mesenteric perfu­sion and bath perfusion).

Outcomes
  • Perfusion pressure
  • Perfusion flow (only for single channel perfusion system)

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