Isolated heart techniques

Isolating the heart is an ex vivo technique used to investigate small mammalians cardiac function, in health and disease, or following drug challenges.

Nowadays, both Langendorff heart and working heart techniques are commonly used. But what is the difference between both techniques?

 

This guide includes:

  • A short video introducing both methods
  • Diagrams and explanations on Working heart and Langendorff techniques
  • Advantages and drawbacks of both techniques
  • Advantages and drawbacks of Constant pressure vs. Constant flow modes, in Langendorff configuration
  • Preffered mode in cardiotoxicity studies

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