This page lists techniques and measurements employed by
the ecgTUNNEL system, and used in the area
of measuring cardiovascular and respiratory outcomes.
The base of the ecgTUNNEL presents 4 silver plates that are connected to electronics and work as contact electrode pads.
A cylinder-shaped tube (the restraining tunnel) restrains the animal firmly and gently, allowing the paws to be in contact with the electrode pads.
Up to 6-lead ECG is recorded and sent to the acquisition software via the USB cable.
ecgTUNNEL is used for more than 10 years for non-invasive ECG recording on rodents.
Non-invasive restrained ECG recording in conscious small rodents: a new tool for cardiac electrical activity investigation – H. Mongue-Din et al. 2007
Effects of mango and mint pod-based e-cigarette aerosol inhalation on inflammatory states of the brain, lung, heart, and colon in mice. Alex Moshensky et al. eLife. 2022
Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations. Caroline Le Dour et al. Nature Communications. 2022
Air volume changes created by a spontaneously breathing, conscious subject within a body box (or plethysmograph) are the basis of the plethysmography technique. These changes are captured using pneumotachographs or pressure transducers, translating into an airflow or pressure signal which is examined and analyzed to gather information on the subject’s pulmonary function.
In the ecgTUNNEL, as the subject breathes spontaneously, the airflow in and out of the body box or the changes in pressure are recorded. The flow signal generated reflects the box pressure changes and is not a direct respiratory flow measurement. Analysis of the waveform follows the subject’s breathing pattern and analyzers within the IOX2 software provide endpoints related to the breathing pattern in general (e.g. respiratory rate, estimates of tidal volume, minute ventilation) or to specific aspects of it (e.g. inspiratory / expiratory time, estimates of peak inspiratory or expiratory flows). The controversial dimensionless quantity known as enhanced pause (Penh) can also be calculated.
The Old and the New: Cardiovascular and Respiratory Alterations Induced by Acute JWH-018 Administration Compared to Δ9 -THC—A Preclinical Study in Mice. Beatrice Marchetti et al. International Journal of Molecular Sciences. 2023
Measuring lung function in mice: the phenotyping uncertainty principle – Bates JH, Irvin CG. J Appl Physiol 94: 1297-1306, 2003.
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