Techniques & Measurements

This page lists techniques and measurements employed by
the ecgTUNNEL system, and used in the area
of measuring cardiovascular and respiratory outcomes.


The base of the ecgTUNNEL presents 4 silver plates that are connected to electronics and work as contact electrode pads.

A cylinder-shaped tube (the restraining tunnel) restrains the animal firmly and gently, allowing the paws to be in contact with the electrode pads.

Up to 6-lead ECG is recorded and sent to the acquisition software via the USB cable.

  • PR, QRS, QT, JT, ST, RR intervals
  • P, T wave durations
  • QTc, JTc corrected values (available with a choice of 6 calculation modes)
  • ST-segment elevation
  • P, Q, R, S, Tneg. & Tpos. amplitudes
  • P, QRS, T wave areas
  • QT in user-adjustable HR ranges
Did you know?

ecgTUNNEL is used for more than 10 years for non-invasive ECG recording on rodents.



Air volume changes created by a spontaneously breathing, conscious subject within a body box (or plethysmograph) are the basis of the plethysmography technique. These changes are captured using pneumotachographs or pressure transducers, translating into an airflow or pressure signal which is examined and analyzed to gather information on the subject’s pulmonary function.

In the ecgTUNNEL, as the subject breathes spontaneously, the airflow in and out of the body box or the changes in pressure are recorded. The flow signal generated reflects the box pressure changes and is not a direct respiratory flow measurement. Analysis of the  waveform follows the subject’s breathing pattern and analyzers within the IOX2 software provide endpoints related to the breathing pattern in general (e.g. respiratory rate, estimates of tidal volume, minute ventilation) or to specific aspects of it (e.g. inspiratory / expiratory time, estimates of peak inspiratory or expiratory flows). The controversial dimensionless quantity known as enhanced pause (Penh) can also be calculated.


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