emkaBATH is a tissue bath setup, designed for contractile measures in tissues such as aortic, tracheal, iliac, and papillary muscles, and/or microvessels such as veins, small arteries, and lymph vessels. The system provides heated, oxygenated, and nutrient-rich perfusate to the tissue with or without drug administration and electrical stimulation.
Tissue baths are widely used to study the effects of agonists and antagonists on tissue contractility. Dose-response curves allow for characterization of a drug’s pharmacological profile and the calculation of EC50 or Emax.
| Specifications | emkaBATH2 | emkaBATH4 |
|---|---|---|
| Max. number of baths | 2 | 4 |
| Bath volumes | 20 ml | 5, 10, 20 ml |
| Bath types | Tubular (PMMA) | Tubular (PMMA), Myograph in option |
| Vessel size | Diameter: 500μm-10mm / Length: 40 mm max | |
| Bath heating regulation | Independent for each bath | Same temperature for all baths |
| Physiologic liquid heating | In line heating of the liquid before entering the bath | |
| Bath temperature range | 20.0-39.0°C ± 0.1°C (68-102.2°F ± 0.18°F) | |
| Hooks | 2 different shapes | 4 different shapes |
| Automatic filling | ||
| Automatic emptying | ||
| Calibration range |
1 scale (user defined, between 0 and 100g) |
3 scales (0-1/0-10/0-50g) |
| Frame size | 300x335x206 mm | 641x500x345 mm |
| Weight | 10 kg | 18 kg |
| Renewed solution by overflow | ||
| Automatic cleaning | ||
| Integrated amplifier | ||
| Bubbling | Frit glass, tube or needle | Frit glass |
| Analog output | 0-5 V | 0-5 V |
| Power supply | 100-240V | 115-230V |
| Transducers | it1, it30 | it1, it30 |
| Positionner resolution |
0.5mm/rotation (manual) |
2.5 μm (0.5mm/rotation; 200 steps/rotation) |
emkaBATH4 can handle:
Contact us to receive some guidelines for tissue bath protocols with atria and papillary muscles, skeletal muscles or smooth muscles from the gastro-intestinal tract.
Tissue bath systems from emka TECHNOLOGIES include automated actions such as baths washes (filling, emptying, overflow), automatic tension… These actions can be manually driven from the device touchscreen, but can also be remotely controlled from IOX software.
Using IOX software also permits to record these actions into the data files and associate them to the physiological data. Each event is displayed on physiological graphs or trends during the acquisition, or in review mode using IOX or Datanalyst software.
In addition, IOX software offers the possibility to write experimental protocols. Such protocols include list of actions (washes, injection, stimulation, calibration, tissues tension…) to be performed on a specific bath, at specific time points or when the user decides.
At the beginning of the experiment, it is not necessary to stay in front of the device during the tissues stabilisation process, as IOX software handles this process automatically.
At last, IOX software controls emka TECHNOLOGIES’ electrical stimulators.
Stimulator start and stop, as well as stimulation parameters (frequency, amplitude, pulse width, number of pulses in a train….) can be adjusted by software and included in the protocol.
emkaBATH4 has been completely redesigned in 2018 and now offers:
