Langendorff & working Heart

Isolated Heart Perfusion system

The isolated heart perfusion system is a versatile solution to investigate small mammalian heart function, in health and disease, or following drug challenges.

Using either the Langendorff or the Working Heart technique, it allows the simultaneous assessment of cardiac electrophysiological and mechanical parameters (ECG, LVP, perfusion flow & pressure, etc.).

The isolated heart perfusion system provides highly reproducible experimental conditions to ensure survival of the isolated heart during several hours.

Compatible Animals

Typical Langendorff & Working Heart Applications

Safety Pharmacology

Toxicology

Heart failure & Cardiac Model development

Arrhythmia

Vascular biology

Cardiotoxicity

Cardiomyopathy

Techniques & Measurements

Langendorff perfusion

Working heart perfusion

Features & Benefits

Modular

Easy to use

Reproducible

Compatible software

IOX2, data acquisition

ecgAUTO, data analysis

Configurations and Extensions

Basic system

The basic system includes the following:

A mainframe: a heart chamber with a heat exchanger, electrovalves, various ports for bolus injection and catheter, and numerous pods for extra attachment (ECG or stimulation electrodes). A Heating jacket is included into the mouse system (in option for rats).
A heating system: thermostat-controlled heater-pumps providing water heated to 37±0.5°C to maintain the isolated heart at a constant temperature and warm perfusion liquid.
A perfusion system to provide oxygen and nutrients to the isolated heart at constant pressure or constant flow: reservoirs, pressure/flow regulator (this regulates pressure or flown and provides signals for flow and pressure too), peristaltic pumps, perfusion cannulas, pressure transducers and perfusion heads (two bubble trap sizes for rat and mouse).
An acquisition system: computer with acquisition software, an amplifier and USB cable.

Extension

Additional transducers may be used to record:

Perfusate temperature
Perfusate flow rate (in working heart mode only)
Electrocardiogram (ECG) by applying contact electrodes to the heart’s surface.

Heating jacket: can be inserted inside the rat heart chamber, to maintain the heart temperature (already included in the mouse system). It includes double-walled glass chamber and plastic parts.

Specifications

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